Polarimetry
The rotation of the plane of polarisation through a sugar solution measured with a half-shade polarimeter and the reaction rate constant for the inversion of cane sugar determined.
- even transparent fluids can change the properties of light passing them
- find out how fast one type of sugar changes into another one
- use optical methods, involving the plane of polarisation, to observe chemical processes
Bath for thermostat, makrolon
External circulation set for thermostat Alpha A
Immersion thermostat Alpha A, 230 V
D (+)-Sucrose 100
Hydrochloric acid 37 %, 1000
Water, distilled 5 l
D(+)-Lactose, powder 100
Crucible tongs, 200 mm, stainless steel
Beaker, 250 ml, low form, plastic
Graduated cylinder 100 ml, PP transparent
Graduated vessel, 1 l, with handle
Funnel, plastic, diameter 100 mm
Spoon, with spatula end, 18 cm, plastic
Glass stirring rod, d = 8 mm, l = approx. 300 mm
Balance OHAUS LG 311, 4 beams, 0…311 g
Tubing connector, ID 6-10mm
Pipette with rubber bulb, long
Stopwatch, digital, 1/100 s
Polarimeter, LED, 590 nm
- To determine the specific rotation of cane sugar (sucrose) and lactose by measuring the rotation of various solutions of known concentration.
- To determine the reaction rate constant when cane sugar is transformed into invert sugar.
- Half-shade principle
- Optical rotatory power
- Optical activity
- Saccharimetry
- Specific rotation
- Reaction rate
- Weber-Fechner law